Solid-Phase Assembly Methods >
Three approaches for assembling DNA fragments encoding
TALE repeat arrays on solid-phase surfaces have been described
in the literature (Reyon
& Tsai et al., Nat Biotechnol. 2012; Briggs
et al., Nucleic Acids Res. 2012; and Wang
et al., Angew Chem Int Ed Engl. 2012). Because
these methods are performed on a solid-phase, they enable
assembly to be carried out using multi-channel pipets or
automated liquid handling robots.
FLASH (Fast Ligation-based
Automatable Solid-phase High-throughput) Assembly
ICA (Iterative Capped Assembly)
Method of Wang et al.