Golden Gate Assembly Methods

A number of groups have described "Golden Gate" methods for assembling DNA encoding TAL effector repeat arrays (Cermak et al., Nucleic Acids Res. 2011; Li et al., Nucleic Acids Res. 2011; Morbitzer et al., Nucleic Acids Res. 2011; Weber et al., PLoS One 2011; Zhang et al., Nat Biotechnol. 2011; Li et al., Plant Mol Biol. 2012). Golden Gate cloning is based on the simultaneous ligation of multiple DNA fragments encoding TAL repeat domains. Reagents and/or software are currently available for practicing three of these platforms and we provide links to these below.

Protocols

For the method of Cermak et al.

For the method of Zhang et al.

Reagents

For the method of Cermak et al.

For the method of Li et al., 2011 (Yang and colleagues)

For the method of Li et al., 2012 (Mahfouz and colleagues)

For the method of Morbitzer et al.

For the method of Weber et al.

Software

For the method of Cermak et al.

For the method of Li et al.

For the method of Zhang et al.

 

 

   

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